![]() Here, orofacial inflammation was induced by injection of complete Freund’s adjuvant (CFA) in mice. However, diverse alterations and regulatory mechanisms following orofacial inflammatory pain in different types of TG neurons remain unclear. Orofacial inflammation leads to transcriptional alterations in trigeminal ganglion (TG) neurons. (J) The percentage of cells expressing CGRP is unchanged in MACS-sorted preparations (n = 4, Fisher's exact test p = 0.229). (H,I) Immunohistochemistry visualizing the neuropeptide calcitonin gene related peptide (CGRP) in unsorted and MACS-sorted preparations. ![]() (G) Percentage of cells positive for Nav1.8 is significantly greater following MACS sorting (n = 4 per condition, Fisher's exact test, *p < 0.05). (E,F) Immunohistochemistry of unsorted and MACS-sorted preparations generated from Nav1.8 TdTomato reporter mice. (D) Cell size distribution of neuronal cells in unsorted and MACS-sorted preparations shows that the MACS-sorted preparation is enriched for small cells <30 μm in diameter (n = 8 per condition. (C) Quantification of neuronal, glial and other non-neuronal cells shows that MACS-sorting generates >95% pure neuronal preparation (n = 8 per condition). (A,B) Immunohistochemistry visualizing neuronal (β-III tubulin), satellite glial (glutamine synthetase) or other non-neuronal (DAPI) cells in unsorted and MACS-sorted neuronal preparations. ![]() Characterisation of MACS-sorted DRG neuronal preparation.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |